Journal: ACS Sensors
Article Title: Smartphone-Based High-Throughput Fiber-Integrated Immunosensing System for Point-of-Care Testing of the SARS-CoV-2 Nucleocapsid Protein
doi: 10.1021/acssensors.2c00754
Figure Lengend Snippet: Optimization of working conditions of HIFS. Optimization of (A) the amount of mAb1 for labeling with PMs, (B) amount of PMs-mAb1, (C) amount of mAb1-HRP, and (D) immunoreaction time. (E) Specificity of HFIS was determined for reacting with 1 μg/mL coronavirus nucleocapsid proteins from SARS-CoV-2, HCoV-229E, HCoV-NL63, HCoV-HKU1, and HCoV-OC43 strains. PBS was used as negative control. (F) Standard curve for quantitative detection of SARS-CoV-2 NP by HFIS. The inset curve presents NP concentrations within 0–4000 pg/mL, of which the linear curve ranges within 0–1000 pg/mL. The data represent the means ± SD of three independent tests. ODR, the optical darkness ratio.
Article Snippet: NPs of coronavirus HCoV-229E, HcoV-NL63, HcoV-HKU1, and HcoV-OC43 strains were purchased from Sino Biological Inc. (Beijing, China) and used for testing of cross reactivity.
Techniques: Labeling, Negative Control